Statistical design and the analysis of gene expression microarray data

M. KATHLEEN KERR, GARY A. CHURCHILL
2001 Genetics Research  
Samples of DNA clones with known sequences are spotted and immobilized onto a glass slide ("microarray"). Pools of purified mRNA from cell populations are reverse-transcribed into cDNA. cDNA is labeled with one of two fluorescent dyes, "red" and "green". Two pools of differentially labeled cDNA are combined and applied to a microarray. REVIEW Strands of cDNA in the pool hybridize to complementary sequences on the array. The "red" and "green" signals from a spot indicate the relative abundance
more » ... the corresponding mRNA in the two cell populations.
doi:10.1017/s0016672301005055 pmid:11355567 fatcat:hwbnpbknq5aktlf3bgfobezu74