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Samples of DNA clones with known sequences are spotted and immobilized onto a glass slide ("microarray"). Pools of purified mRNA from cell populations are reverse-transcribed into cDNA. cDNA is labeled with one of two fluorescent dyes, "red" and "green". Two pools of differentially labeled cDNA are combined and applied to a microarray. REVIEW Strands of cDNA in the pool hybridize to complementary sequences on the array. The "red" and "green" signals from a spot indicate the relative abundancedoi:10.1017/s0016672301005055 pmid:11355567 fatcat:hwbnpbknq5aktlf3bgfobezu74