Induction of cell-mediated immunity to Mycobacterium leprae in mice

P J Patel, M J Lefford
1978 Infection and Immunity  
The immune response of mice to armadillo-derived, irradiation-killed Mycobacterium leprae (I-ML) was investigated. Following injection of 100 jig of I-ML into the left hind footpads of mice, a state of cell-mediated immunity (CMI) was engendered to antigens of M. leprae. The evidence for CMI was as follows: (i) development of delayed-type hypersensitivity to both human tuberculin purified protein derivative and soluble M. leprae antigens; (ii) T-lymphocyte-dependent macrophage activation at the
more » ... e activation at the inoculation site; (iii) specific systemic resistance to the cross-reactive species M. tuberculosis; and (iv) immunopotentiation of the delayed-type hypersensitivity response to an unrelated antigen. The CMI induced by I-ML in aqueous suspension was greater than that obtained with the same antigen in water-in-oil emulsion, even though the latter generated a more severe reaction at the site of immunization. I-ML also induced a stronger CMI response than the corresponding dose of heat-killed BCG. was provided by R. J. W. Rees at a concentration of 610 pg of protein per ml. It was appropriately diluted 87 on May 9, 2020 by guest Downloaded from
doi:10.1128/iai.19.1.87-93.1978 fatcat:nin4ov5nbfcsll66fb43v5ts3m