The exploration and use of lipases produced by microorganisms are in line with societal concerns since they provide clean development options within the standards of environmental sustainability. The objective of this work was therefore to isolate microorganisms with the potential of producing lipases, which could be used in the waste water treatment systems of slaughterhouse units. Waste water samples were collected from the treatment lagoons of a poultry slaughterhouse. The PCA culture medium

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... was used for the isolation of fungi. The pH was adjusted to 6.5 and autoclaved at 1 atm of pressure, for a time of 30 min. The isolated and purified colonies were evaluated in order to obtain lipase producing strains. The results obtained in this study enabled the conclusion that the waste water produced in slaughterhouse has a high microbial load, highlighting bacteria with 6.8 × 10 8 , filamentous fungi with 3.4 × 10 4 and yeasts with 2.2 x 10 2 CFU.ml -1 . Out of this microbial population, six species of lipase producing fungi were isolated, with the fungi Aspergillus flavus and Aspergillus terreus being those that obtained the highest enzymatic indices.