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PCR Instrument-assisted Acidolysis for Monosaccharide Composition Analysis of Serum Glycans [post]

Yiran Zhang, Meng Zhang, Lijuan Zhang
2021 unpublished

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This protocol describes the procedures where a PCR instrument-assisted acidolysis is used for releasing monosaccharides from serum glycans. The monosaccharide composition analysis is subsequently obtained by a HPLC method that separates and quantifies all 1-phenyl-3-methyl-5-pyrazolone (PMP)-labeled monosaccharides in 10 μl serum in 20 minutes. The rapid heating, precise temperature control, and gradient heating properties of PCR instrument provides with consistent acidolysis and derivatization
more » ... conditions for up to 96 samples simultaneously. The described workflow takes approximately 4–5 h, up to 72 serum samples can be analyzed with one HPLC instrument per day.
doi:10.21203/rs.3.pex-1280/v1 fatcat:dvc2rsbzpzbqhbfl2lmipczwzm
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Yiran Zhang, Meng Zhang, Lijuan Zhang. "PCR Instrument-assisted Acidolysis for Monosaccharide Composition Analysis of Serum Glycans." (2021)