AN EMBRYOLOGICAL STUDY OF MOUSE EAR USING THE WHOLE EMBRYO CULTURE TECHNIQUE

TOMOAKI SUGIURA
1993 Nippon Jibiinkoka Gakkai Kaiho  
Mouse embryos were explanted at 11.5 days of gestation and cultured for periods of 6 , 12, 18, 24, 30, 36, 42 and 48 hours using the whole embryo culture system. A continuous flow of gas (5% CO2+95%O2) was maintained through the culture bottles during the culture periods. The cultured embryos were morphologically and histologically examined at the end of the designated periods . The growth of the embryos was depressed after about 36 hours, and differentiation was retarded as the culture time
more » ... the culture time increased. General development had ceased almost completely by 42-48 hours . At 18-36 hours, the external auditory canal and pinna, which are not developed in 11.5 day embryos ,
doi:10.3950/jibiinkoka.96.665 fatcat:omnpgn6jefeenadfcdy5dv7wri