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Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos
[chapter]
2020
Msphere
Preserved Fulltext
Small RNAs mediate posttranscriptional gene silencing in plants and animals. This often occurs in specific cell or tissue types and can be necessary for their differentiation. Determining small RNA (sRNA) localization patterns at cellular resolution can therefore provide information on the corresponding gene regulatory processes they are involved in. Recent improvements with in situ hybridization methods have allowed them to be applied to sRNAs. Here we describe an in situ hybridization
doi:10.1007/978-1-0716-0342-0_7
pmid:31975297
fatcat:m4hxfds7xnfj7oonminaqtvmfy