Improving the Reliability, Ease, and Efficiency of Section Staining in a Diagnostic Laboratory with the mPrep/g System for TEM Grid Processing

Craig Radi
2015 Microscopy and Microanalysis  
In a clinical diagnostic lab it is important to obtain results efficiently. With Transmission Electron Microscopy (TEM), a common problem is inconsistent staining of tissue sections that can lead to inefficiency. Poor staining can substantially delay diagnoses due to the time to prepare more sections, stain more grids, and then repeat TEM imaging. Most labs have a favorite method, with many using a variation of droplet staining [1]. We recently learned of an entirely new method based on mPrep/g
more » ... (microscopy preparation for grids) capsules that can simultaneous stain 16 or more grids. In the present study we compare the mPrep/g method to our droplet method.
doi:10.1017/s1431927615004420 fatcat:5sknzuoi4jbzbbgk4kt6t46n2e