The Interaction of Blood Flow, Insulin, and Bradykinin in Regulating Glucose Uptake in Lower-Body Adipose Tissue in Lean and Obese Subjects
Journal of Clinical Endocrinology and Metabolism
Context: Impaired adipose tissue (AT) blood flow has been implicated in the pathogenesis of insulin resistance in obesity. Insulin and bradykinin are meal-stimulated promoters of AT blood flow and glucose metabolism. Objective: We tested whether blood flow regulates glucose metabolism in AT, insulin and bradykinin exert additive effects on AT blood flow and metabolism, and any of these actions explains the insulin resistance observed in obese individuals. Design: Perfusion and glucose
... d glucose metabolism in the AT of the thighs were studied by positron emission tomography and H 2 15 O (flow tracer) and 18 F-2-fluoro-2-deoxyglucose. Study I included five subjects in whom positron emission tomography imaging was performed in the fasting state during intraarterial infusion of bradykinin in the left leg; the right leg served as a control. Study II included seven lean and eight obese subjects in whom the imaging protocol was performed during euglycemic hyperinsulinemia. Results: Bradykinin alone doubled fasting AT blood flow without modifying glucose uptake. Hyperinsulinemia increased AT blood flow (P Յ 0.05) similarly in lean and obese individuals. In the lean group, bradykinin increased insulin-mediated AT glucose uptake from 8.6 Ϯ 1.6 to 12.3 Ϯ 2.4 mol/min ⅐ kg (P ϭ 0.038). In the obese group, AT glucose uptake was impaired (5.0 Ϯ 1.0 mol/ min ⅐ kg, P ϭ 0.05 vs. the lean group), and bradykinin did not exert any metabolic action (6.0 Ϯ 0.8 mol/min ⅐ kg, P ϭ 0.01 vs. the lean group).