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Measurement of Serum Superoxide Dismutase Activity by Electron Spin Resonance

Haruo MIYAGAWA, Toshikazu YOSHIKAWA, Toru TANIGAWA, Norimasa YOSHIDA, Shigeru SUGINO, Motoharu KONDO, Hiroshige NISHIKAWA, Masahiro KOHNO
1988 Journal of clinical biochemistry and nutrition  

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We measured serum superoxide dismutase activity by electron spin resonance, using 100 m1vl 5, 5-dimethyl-l -pyrroline-N-oxide (DMPO) as a trap. The addition of superoxide dismutase decreased the height of the DMPO-OOH signal produced by the hypoxanthine-xanthine oxidase system. Serum superoxide dismutase activity was determined from a quantitative line for superoxide dismutase. When bovine Cu,Znsuperoxide dismutase (50,000 U/kg) was injected intravenously into rats, the superoxide dismutase
more » ... vity decreased almost linearly with a half life of about 6 min. This method allowed direct detection of superoxide radicals and new measurement of superoxide dismutase activity.
doi:10.3164/jcbn.5.1 fatcat:p5jgqm7ecrd3tnpqowlytbnqtq
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Haruo MIYAGAWA, Toshikazu YOSHIKAWA, Toru TANIGAWA, Norimasa YOSHIDA, Shigeru SUGINO, Motoharu KONDO, Hiroshige NISHIKAWA, Masahiro KOHNO. "Measurement of Serum Superoxide Dismutase Activity by Electron Spin Resonance." Journal of clinical biochemistry and nutrition 5.1 (1988) 1-7