Adaptation of a coculture technique to the Minitek anaerobe system

Z Hussain, R Lannigan, H Bürger, D Groves
1985 Journal of Clinical Microbiology  
A method to produce anaerobic conditions by coculture with a nonfermentative organism was utilized in conjunction with the Minitek anaerobe system (BBL Microbiology Systems, Cockeysville, Md.) for identification of anaerobic bacteria from clinical specimens. With the coculture method, the Minitek anaerobe identification tests could be incubated under aerobic conditions. In 1,900 individual biochemical reactions, 1,826 (96%) were identical whether anaerobic conditions were achieved by
more » ... ieved by conventional or coculture techniques. In comparison with the reference identification (Virginia Polytechnic Institute and State University, Blacksburg), both systems of incubation identified 91 of 99 strains (92%) correctly. The method of incubation had an effect on identification to the genus level in 1 of 99 (1 %) strains and to the species level in 3 of 99 (3%) strains. A method of conveniently prQviding reducing conditions for anaerobic culture through coculture with Acinetobacter lwoffli has been described for use in biochemical tests performed in conventional tubed media (3). Microtube methods are commercially available for identification of anaerobes, and their use is widespread despite some limitation in their application (1, 4, 5) . This study describes the adaptation of coculture incubation to the Minitek anaerobe (BBL Microbiology Systems, Cockeysville, Md.) microtube system and a comparison of coculture and conventional incubation results with reference identifications obtained with prereduced anaerobically sterilized media (Virginia Polytechnic Institute and State University, Blacksburg [VPI]) (2). Bacterial strains. The 102 organisms used in this study were clinical isolates from the Regional Anaerobe Laboratory in St. Joseph's Hospital, Hamilton, Ontario, or from Victoria Hospital, London, Ontario. All were fresh isolates or were kept frozen after minimum subculture. The frozen isolates were subcultured three or four times before being tested. The strains tested in this study were as follows: 4
doi:10.1128/jcm.21.4.645-646.1985 fatcat:64q6fbasbzawfhseczn2n7flhy