Contribution to the Study of Antibiotic Resistance on Salmonella and Shigella Strains Isolated in Central African Republic

Chrétien Djimeli
2017 Journal of Microbiology & Experimentation   unpublished
Peoples are contaminated by germs such as Salmonella and Shigella either from the environment or by contact with infected animals on the farm. They are frequently infected by the consumption of raw or undercooked meat, contaminated during the slaughter process. These germs are the leading cause of gastroenteritis food borne humans cause symptoms of a wide range of severity, usually manifested by a more or less severe diarrhea sometimes requiring therapeutic management. Objectives: This
more » ... ives: This prospective study carried out over the period from July 2011 to June 2012 aims at evaluating the prevalence of Salmonella and Shigella strains and determine their resistance to common antibiotics, usually available in Central African Republic markets. Methods: The collection of sheets of samples were used to collect the data, the technique of the conventional bacteriology was used. The isolated bacteria were identified by the API 20E and LEMINOR galleries. The agar diffusion technique was used to determine the antibiotic resistance strains. Results: Amongst the 2500 samples, 72 cases of Salmonella and 182 cases of Shigella were registered. Amongst the 72 cases of Salmonella isolated, 38 cases occur in female patients against 27 in male and 7 unspecified cases. Likewise, amongst the 182 cases of Shigella registered, 89 cases occur in female patients against 75 cases in male and 18 undetermined cases. Identification tests revealed that different Salmonella isolated were Salmonella paratyphi A and Salmonella arizonae. Likewise, among the Shigella strains isolated, identification tests revealed Shigella flexneri and Shigella dysanteriae. Antibiotherapy showed that Salmonella strains were resistant to tetracycline, cotrimoxazole, and amoxicillin and sensitive to ceftriaxone, cefotaxime and ciprofloxacin. Likewise, those of Shigella were resistant to cotrimoxazole, amoxicillin and chloramphenicol and sensitive to ceftriaxone, cefotaxime and ciprofloxacin. Conclusion: This study revealed that, although both infections were frequent, there was more Salmonella infection than Shigella infection. Salmonella and Shigella infect much the age group of 0-5 years and 25 and over. The Salmonella spp and Shigella spp were the most isolated strains.