PTU-072 Evaluation of the Impact of different Commercially available DNA Extraction Kits and Laboratories for Assessing Bacterial Community Structure in Faecal Samples – Implications for IBD Research

N Kennedy, A Walker, S Berry, S Duncan, F Farquharson, P Louis, J Satsangi, H Flint, C Lees, G Hold
2013 Gut  
Conclusion This study demonstrates significant differences in DNA yield and bacterial DNA composition seen when comparing DNA extracted from the same faecal sample with different extraction kits. This highlights the importance of ensuring that all samples to be analysed together are prepared with the same DNA extraction method, and the need for caution when comparing studies that have used different methods.
doi:10.1136/gutjnl-2013-304907.164 fatcat:nsrqbiljejgjrbbhpjsr7ufk54