PRODUCTION OF CARBON DIOXIDE IN VITRO BY BLASTOCYSTS FROM INTACT AND OVARIECTOMIZED MICE

C. A. TORBIT, H. M. WEITLAUF
1975 Reproduction  
N ormal' and'd elayed' implanting' mouse blastocysts were incubated in non-radioactive culture medium for various lengths of time (preincubation), and then placed in culture medium with [ 14C] \ x=r eq-\ glucose for 2 hr. The rate of embryonic CO2 production was estimated from 14CO2 given off by the blastocysts. Normal embryos were unaffected by the length of preincubation. By contrast,'d elayed implanting' embryos had a low level of CO2 production with short preincubations (i.e. less than 2
more » ... , but approached the normal range with longer preincubations (i.e. more than 8 hr) and thus, were'activated' in vitro. Incubation of the'd elayed implanting' embryos with actinomycin D prevented the expected increase in CO2 production, indicating that synthesis of new RNA is necessary for their activation. Preincubation of normal and'd elayed implanting' blastocysts with oestradiol-17\g=b\ and/or progesterone had no effect on the level of CO2 production, suggesting that the hormones do not directly stimulate or inhibit carbohydrate metabolism in the embryos.
doi:10.1530/jrf.0.0420045 pmid:1167385 fatcat:ehmuzlgsbve2dhwsciyebbtafq