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Sequential primed kinases create a damage-responsive phosphodegron on Eco1
2013
Nature Structural & Molecular Biology
Sister-chromatid cohesion is established during S phase when Eco1 acetylates cohesin. In budding yeast, Eco1 activity falls after S phase due to Cdk1-dependent phosphorylation, which triggers ubiquitination by SCF Cdc4 . We show here that Eco1 degradation requires the sequential actions of Cdk1 and two additional kinases, Cdc7-Dbf4 and the GSK-3 homolog Mck1. These kinases recognize motifs primed by previous phosphorylation, resulting in an ordered sequence of three phosphorylation events on
doi:10.1038/nsmb.2478
pmid:23314252
pmcid:PMC3565030
fatcat:7jlyv5xszvg5ta6uczyord46jy