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Biodiesel usually synthesized by transesterification of triglyceride and alcohol by addition of acid or base catalyst so there is could produce a waste of chemical process. Alternative process is by using biocatalyst such as enzyme to synthesize biodiesel without chemical process waste. In this research, synthesis of biodiesel from Crude Palm Oil (CPO) that through the process of degumming and methyl acetate as acyl donor has been investigated with using Lipozyme as biocatalyst. Variables indoi:10.12777/ijse.7.2.174-177 fatcat:rb5lmbnllbgbjg34yxw35outwa