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A platform for efficient transgenesis inMacrostomum lignano, a flatworm model organism for stem cell research [article]

Jakub Wudarski, Daniil Simanov, Kirill Ustyantsev, Katrien de Mulder, Margriet Grelling, Magda Grudniewska, Frank Beltman, Lisa Glazenburg, Turan Demircan, Julia Wunderer, Weihong Qi, Dita B. Vizoso (+8 others)
2017 bioRxiv   pre-print

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Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration and tissue patterning. However, the lack of transgenesis methods significantly hampers their wider use. Here we report development of a transgenesis method forMacrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently
more » ... results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated theM. lignanogenome, including genome-wide mapping of transcription start regions, and showed its utility by generating multiple stable transgenic lines expressing fluorescent proteins under several tissue-specific promoters. The reported transgenesis method and annotated genome sequence will permit sophisticated genetic studies on stem cells and regeneration usingM. lignanoas a model organism.
doi:10.1101/151654 fatcat:n3c7yjib75aqjpkeuzsllpkapm
Citation

Jakub Wudarski, Daniil Simanov, Kirill Ustyantsev, Katrien de Mulder, Margriet Grelling, Magda Grudniewska, Frank Beltman, Lisa Glazenburg, Turan Demircan, Julia Wunderer, Weihong Qi, Dita B. Vizoso, Philipp M. Weissert, Daniel Olivieri, Stijn Mouton, Victor Guryev, Aziz Aboobaker, Lukas Scharer, Peter Ladurner, Eugene Berezikov. "A platform for efficient transgenesis inMacrostomum lignano, a flatworm model organism for stem cell research." bioRxiv (2017)