As nutritional factors affecting the salt tolerance of Staphylococcus epidermidis, the requirement for vitamin and amino acid was tested using the standard media containing NaCl and/or Na-cholate. When Na-cholate was added to the standard medium, the requirement for riboflavin was increased. On the other hand, the requirement for p-aminobenzoic acid, folic acid and pantothenic acid was increased by the addition of NaCl. Furthermore, when NaCl and Na-cholate were added, pantothenic acid and

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... tol were essential and also folic acid was necessary for the normal growth of this organisms. Regarding to amino acid requirement, the addition of both NaCl and Na-cholate increased the requirement for histidine, proline, glycine and tyrosine, but the requirement for threonine was decreased.