Mirasol PRT system inactivation efficacy evaluated in platelet concentrates by bacteria-contamination model

Miodrag Jocic, Miroljub Trkuljic, Dragana Jovicic, Nemanja Borovcanin, Milena Todorovic, Bela Balint
2011 Vojnosanitetski Pregled  
Background/Aim. Bacterial contamination of blood components, primarily platelet concentrates (PCs), has been identified as one of the most frequent infectious complications in transfusion practice. PC units have a high risk for bacterial growth/multiplication due to their storage at ambient temperature (20 ± 2°C). Consequences of blood contamination could be effectively prevented or reduced by pathogen inactivation systems. The aim of this study was to determine the Mirasol pathogen reduction
more » ... athogen reduction technology (PRT) system efficacy in PCs using an artificial bacteria-contamination model. Methods. According to the ABO blood groups, PC units (n = 216) were pooled into 54 pools (PC-Ps). PC-Ps were divided into three equal groups, with 18 units in each, designed for an artificial bacteria-contamination. Briefly, PC-Ps were contaminated by Staphylococcus epidermidis, Staphylococcus aureus or Escherichia coli in concentrations 10 2 to 10 7 colony forming units (CFU) per unit. Afterward, PC-Ps were underwent to inactivation by Mirasol PRT system, using UV ( = 265-370 nm) activated riboflavin (RB). All PC-Ps were assayed by BacT/Alert Microbial Detection System for CFU quantifica-Volumen 68, Broj 12 Jocić M, et al. Vojnosanit Pregl 2011; 68(12): 1041-1046. posle kontaminacije bakterijama. Uzorci iz jedinica tretiranih Mirasolom ispitivani su na prisustvo CFU jedan sat, odnosno 3 i 5 dana nakon inaktivacije. Rezultati. Tokom perioda čuvanja/istraživanja postignuta je kompletna inaktivacija bakterija svih vrsta u koncentracijama od 10 2 i 10 3 CFU po P-KT. Najefikasnija inaktivacija (10 5 CFU po P-KT) postignuta je pri ispitivanju bakterije Escherichia coli. Nasuprot tome, inaktivacija kod sve tri vrste bakterija nije bila efikasna u koncentracijama bakterija ≥ 10 6 CFU po P-KT. Zaključak. Efikasna inaktivacija ispitivanih bakterija sa bitnim smanje-njem CFU u P-KT -3 Log postignuta je za sve tri vrste bakterija i 5 Log za bakteriju Escherichia coli. Bezbednost terapije krvnim komponentama, prvenstveno klinička primena KT, može biti unapređena korišćenjem sistema Mirasol PRT. Ključne reči: trombociti; transfuzija trombocita; infekcija, bakterijska; lečenje, ishod; vitamin b2; ultravioletni zraci.
doi:10.2298/vsp1112041j pmid:22352265 fatcat:wvgcssnmzbf3foeyk5krrsg4iq