Apoptosis Induced By Low-Concentration Ethanol In Hepatocellular Carcinoma Cell Strains And Down-Regulated Afp And Survivin Analysis By Proteomic Technology

Xin Kai, Juan Li, Sexin Huang, Zengliang Bai
2010 Zenodo  
Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first
more » ... were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.
doi:10.5281/zenodo.1333346 fatcat:u7zkixgihzbo3pdzn6niirl4d4