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Three-dimensional (3D) tissue culture provides a physiologically relevant microenvironment for distinguishing malignant from non-malignant breast cell phenotypes. 3D culture assays can also be used to test novel cancer therapies and predict a differential response to radiation between normal and malignant cells in vivo. However, biological measurements in such complex models are difficult to quantify and current approaches do not allow for in-depth multifaceted assessment of individual coloniesdoi:10.1039/c3ib20275e pmid:23407655 pmcid:PMC3641787 fatcat:lgnyiuyo6zdjpihlukkeuybpkm