Oxidative stress plays an essential role in the normal regulation of various cellular physiological processes. It is known to contribute to the pathogenesis of lung cancer as the leading cause of cancer worldwide. While platinum-based chemotherapy has been the standard of care in advanced lung cancer, we hypothesised that platinum nanoparticles (PtNPs) can play a role in reversing oxidative stress in human A549 epithelial lung cell lines. Hydrogen peroxide (H2O2) induces oxidative stress in

more »

... s and the role of PtNPs was determined to lower the capacity in metabolising H2O2. The oxidative status was assessed through antioxidant enzyme activity and DNA damage was qualitatively and quantitatively measured through comet assay. Even though the antioxidant capacity of the PtNPs was found to be significantly lower than ascorbic acid through the ferric reducing antioxidant potential (FRAP) assay, the scavenging of free radical activity of PtNPs is still durable at high concentrations. PtNPs were found to be able to reduce H2O2-induced DNA damage significantly by increasing antioxidant enzyme activity such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAS). Hence, PtNPs could be a promising antioxidant in the treatment of oxidative stress-related conditions, specifically lung cancer.