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(1) Background: The COVID-19 epidemic had caused more than 100 million confirmed cases worldwide by the end of January 2021. The focus of this study was to explore which stress was felt the most by nursing staff in isolation wards in the face of dangerous infectious diseases. (2) Methods: Nursing staff in negative pressure isolation wards were taken as the research objects. The sources of stress were divided into 14 items in three categories, namely, patient care, infection protection, anddoi:10.3390/healthcare9040462 pmid:33919871 fatcat:yvdmysgsazd7jinexb5lgt3nby