Procedures that have been developed for the purification of acetylornithine 8transaminase from Escherichia coli W also lead to the simultaneous purification of ornithine 8-transaminase. These two enzymatic activities have the same electrophoretic mobility and are identical immunochemically. Studies of inhibition kinetics demonstrate that the two substrates, acetylornithine and ornithine, compete for the same active site of acetylornithine 8-transaminase; thus, the ornithine 8-transaminase

more »

... ty in E. coli is due to acetylornithine 8transaminase and not to a separate specific ornithine 8-transaminase. on May 9, 2020 by guest