The Value of Alveolar Lavage Fluid mNGS in The Peripheral Pulmonary Among Children [post]

Kun-yin Qiu, Xiong-yu Liao, Jian-pei Fang, Dun-hua Zhou
2021 unpublished
Objectives The aim of this study was to evaluate the value of alveolar lavage fluid mNGS in the diagnosis of peripheral pulmonary infectious lesions among children. Methods Twenty-eight consecutive pediatric patients suspected of pulmonary infections were retrospectively. Result The detection rate of traditional pathogen method was 17.9%, while the detection rate of mNGS was 71.4% (P < 0.01,). The smoothing curve also showed that, there is a negative linear correlation between WBC and microbial
more » ... n WBC and microbial reads (P < 0.05,). Further we found when CRP is less than 60mg/dl, CRP increases with the increase of microbial sequencing reads. With the ROC curves, we demonstrated the different detection methods of alveolar lavage fluid in the diagnosis of peripheral pulmonary infectious lesions.The results showed that mNGS was more sensitive (100%) and specific (90.9%) compared to traditional method (83.3%, 18.2%). The PPV and NPV of mNGS were 100% and 75%. The AUC of mNGS was 95.45%, while the AUC of traditional methods was 50.76%, which was with a significant difference (P < 0.001). The PPV and NPV of traditional pathogen detection were 80% and 21.7%. Conclusions Metagenomic NGS analysis can provided fast and precise pathogen detection and identification among children, and WBC and CRP could be used biomarkers of anti-infective efficacy.
doi:10.21203/rs.3.rs-150661/v1 fatcat:hzffnf4c5fdvvbchgoayoqzed4