PNA-Mediated Purification of PCR Amplifiable Human Genomic DNA from Whole Blood

Corina Seeger, Hans-Georg Batz, Henrik Ørum
1997 BioTechniques  
A simple and rapid procedure whereby human genomic DNA can be purified in a PCR amplifiable form from whole blood is described. In a first step, human genomic DNA is hybridized in solution to a biotinylated peptide nucleic acid (PNA), which forms a high-affinity triplex with A 7 sequence motifs in the target DNA. The complex is then captured onto paramagnetic streptavidin-coated particles, which are subsequently transferred directly into the PCR. The purification method effectively removes
more » ... tively removes inhibitors of the PCR from as much as 500 µ L of whole blood.
doi:10.2144/97233rr02 pmid:9298225 fatcat:fya3nliddncejg5xvqvxh7n2nu