Antitubercular Activity and Inhibitory Effect on Epstein-Barr Virus Activation of Sterols and Polyisoprenepolyols from an Edible Mushroom, Hypsizigus marmoreus

Toshihiro Akihisa, Scott Gary Franzblau, Harukuni Tokuda, Masaaki Tagata, Motohiko Ukiya, Tsunetomo Matsuzawa, Koichi Metori, Yumiko Kimura, Takashi Suzuki, Ken Yasukawa
2005 Biological and Pharmaceutical Bulletin  
Seven sterols (1-7) and eight polyisoprenepolyols (8-15), isolated from the non-saponifiable lipid fraction of the dichloromethane extract of an edible mushroom, Hypsizigus marmoreus (Buna-shimeji), were tested for their antitubercular activity against Mycobacterium tuberculosis strain H 37 Rv using the Microplate Alamar Blue Assay (MABA). Six sterols (2-7) and two polyisoprenepolyols (8, 12) showed a minimum inhibitory concentration (MIC) in the range of 1-51 m mg/ml, while the others (1,
more » ... the others (1, 9-11, 13-15) were inactive (MICϾ128 m mg/ml). The seven sterols (1-7) and three polyisoprenepolyols (8, 10, 12) were further evaluated for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells. Sterols 6 and 7 showed potent inhibitory effects while preserving the high viability of Raji cells.
doi:10.1248/bpb.28.1117 pmid:15930759 fatcat:qwdf64js3vg2hofariui4bmtym