Induction and Analysis of Synchronous Meiotic Yeast Cultures: Figure 1

G. Valentin Börner, Rita S. Cha
2015 Cold Spring Harbor Protocols  
Meiosis in Saccharomyces cerevisiae can be induced by deprivation of nutrients. Here, we present a protocol for inducing synchronous meiosis in SK1, the most efficient and synchronous yeast strain for meiosis, by exposing SK1 cells to liquid medium that contains potassium acetate as a nonfermentable carbon source and lacks nitrogen. These synchronous meiotic yeast cultures can be subjected to a range of molecular and cytological analyses, making them useful for investigating the genetic and
more » ... the genetic and molecular determinants of meiosis. MATERIALS It is essential that you consult the appropriate Material Safety Data Sheets and your institution's Environmental Health and Safety Office for proper handling of equipment and hazardous material used in this protocol. RECIPES: Please see the end of this protocol for recipes indicated by . Additional recipes can be found online at http://cshprotocols.cshlp.org/site/recipes. YPD plates YPG plates Equipment Centrifuge (with rotors and sterile centrifuge tubes ranging in sizes from 15 to 500 mL depending on the culture volume) Coverslips for microscope slides (No. 1.5; 20 × 20 mm)
doi:10.1101/pdb.prot085035 pmid:26430251 fatcat:pl4mrby5zrawtlhl6bop5b5kqy