Physiological effects of a bactericidal protein from human polymorphonuclear leukocytes on Pseudomonas aeruginosa

C J Hovde, B H Gray
1986 Infection and Immunity  
The physiological changes seen in Pseudomonas aeruginosa after exposure to a bactericidal protein (BP) from the granules of human polymorphonuclear leukocytes were studied. It was demonstrated, using radiolabeled proline or leucine, that both the rate of cellular uptake and amino acid incorporation into trichloroacetic acid-insoluble material were markedly decreased immediately after exposure to BP. The rate of 02 consumption by P. aeruginosa was decreased immediately after exposure to BP and
more » ... ntinued to decline exponentially until it ceased completely 30 min after exposure to BP. In the presence of 30 mM CaC12 or MgC12, bacteria were protected from death due to BP and respiration rates were unaffected. The cellular ATP pool of P. aeruginosa remained constant for up to 2 h after exposure to BP. Membrane depolarization was measured by the influx of the lipophilic anion thiocyanate. It was shown that the cytoplasmic membrane of P. aeruginosa was partially depolarized after exposure to BP. Purified BP killed 95% of 5 x 106 CFU of P. aeruginosa at a concentration of 60 to 100 ng of protein per ml. Although the concentration of bacteria and BP varied with each type of experiment, the BP/bacteria ratio required to cause a 95 to 99% loss in viability remained constant. We propose that cytoplasmic membrane depolarization is the biochemical lesion responsible for the other 90
doi:10.1128/iai.52.1.90-95.1986 fatcat:xdgkdkkgyjetdpwufvth2dyhqi