New methods for rapid detection of aflatoxin

C. W. Hesseltine, O. Shotwell
1973 Pure and Applied Chemistry  
The rapid detection of mycotoxin contamination in agricultural commodities is an area of research that requires more attention. The many companies buying hundreds of bushels of cereal grains daily, need to inspect their purchases immediately and to make a decision in minutes. Arising from this need to detect aflatoxin, we have developed two methods for rapid identification of aflatoxin in corn. The first is based upon a glowing greenish-gold fluorescence produced under ultraviolet light (365
more » ... iolet light (365 nm) by corn kernels that contain aflatoxin. The fluorescent material is not aflatoxin hut a compound associated with it. Fluorescence depends upon the interaction between enzymes in corn and a compound produced by members of the Aspergillusfiavus series. Heatsterilized corn inoculated with A. parasilicus does not fluoresce, although aflatoxin is formed. Fluorescence is seen in broken kernels but it is not visible in intact corn kernels until they are broken. Laboratory studies show that this method of detection is applicable for other cereals, including wheat, rice, oats and barley, but is apparently not effective for soybeans and peanuts. The second is a modification of the chromatographic mini-column method devised for corn.
doi:10.1351/pac197335030259 fatcat:gh7arjzcz5bareaowmd3mevgt4