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Salt concentration governs nucleic acid hybridization according to the Schildkraut-Lifson equation. High concentrations of SDS are used in some common protocols, but the effects of SDS on hybridization stringency have not been reported. We investigated hybridization parameters in solutions containing SDS. With targets immobilized on nylon membranes and PCR-or transcription-generated probes, we report that the 50% dissociation temperature (T m *) in the absence of SDS was 15°C-17°C lower thandoi:10.2144/02331st01 pmid:12139257 fatcat:bzt3nk45t5gfrkc2j2d56pf4cu