EXOGENOUS ADMINISTRATION OF L-ARGININE ENHANCES THE ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA (BLACKSEED) OIL IN WISTAR RATS

Abdulbasit Amin, Bamidele Owoyele
2014 Archives •   unpublished
Nigella sativa (NS) Linn. (Ranunculaceae) oil is used in Northern Nigeria for the treatment of malaria, stomach ulcer, and other inflammatory diseases. The effect of NS (NS) oil on inflammation and the nitric oxide (NO) system was investigated in this study. Chronic inflammation was induced using formaldehyde arthritic model while acute inflammation was produced using carrageenan-induced paw oedema in rats. For the chronic inflammatory model, the animals were divided into four groups of five
more » ... r groups of five animals each as follows: Group A, B, C, and D, they were administered normal saline, indomethacin (5 mg/Kg), NS oil (1 ml/Kg), and NS oil (2 ml/Kg) orally respectively, while the acute inflammatory model had a fifth group, E, administered N G-nitro-L-arginine methyl ester (L-NAME, 100 mg/Kg). In order to investigate the effect of L-arginine administration, the acute inflammation experiment was repeated with intraperitoneal administration of L-arginine (300 mg/kg) to all groups 1h after the induction of inflammation. Oedema (inflammation) was measured daily for the 10-day duration of the chronic inflammatory model and hourly for the 6h duration of the acute inflammatory model. The results showed that NS oil has the capacity to inhibit acute and chronic inflammation; it also showed that administration of L-arginine enhanced the anti-inflammatory activity of NS oil. It is hereby concluded that the anti-inflammatory activity of NS oil may be enhanced by L-arginine.
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