Structural analysis and transcriptional mapping of the Marek's disease virus gene encoding pp38, an antigen associated with transformed cells

Z Z Cui, L F Lee, J L Liu, H J Kung
1991 Journal of Virology  
The gene encoding a Marek's disease virus (MDV) pp38 phosphoprotein has been identified, sequenced, and localized to the BamHI H fragment to the left of the putative MDV origin of replication. The open reading frame was defined by sequencing of a lacZ-pp38 fusion protein gene from a Agt 1 expression library. The entire open reading frame is 290 amino acids long and codes for a protein with a calculated molecular weight of 31,169, compared with the size of 38 kDa of the phosphorylated form
more » ... ted by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Si nuclease protection analysis showed that the pp38 gene is transcribed leftward as an unspliced mRNA. On the basis of transcriptional mapping studies, the pp38 transcript is predicted to be about 1.8 kb in length without a poly(A) sequence. Its promoter-enhancer region overlaps that of the major rightward BamHI H 1.8-kb transcript implicated in tumor induction. This region VOL. 65, 1991 on May 9, 2020 by guest
doi:10.1128/jvi.65.12.6509-6515.1991 fatcat:ptchpt66bzgclkgeqgx2omuzny