Development and validation of RP-HPLC-PDA method for the analysis of Citalopram hydrobromide in bulk, dosage forms and in in vitro dissolution samples

Ch Kumar, A Reddy, G Suneetha, M Sree, M Lakshmi, Buchi Nalluri
2014 Journal of Chemical and Pharmaceutical Research   unpublished
The objective of the current work is to develop a simple, efficient, economical and LC-MS compatible RP-HPLC-PDA method for the analysis of Citalopram Hydrobromide in bulk, dosage forms and in dissolution samples. Samples were separated on Agilent Eclipse C 18 column (150 x 4.6mm, 5µm) with mobile phase composed of 10mM Ammonium acetate: methanol (35:65 v/v) in isocratic mode. The detection wavelength was fixed at 239nm. The retention time of Citalopram Hydrobromide was 3.1min and the method
more » ... n and the method showed a good linearity in the concentration range of 5-25µg/mL with a correlation coefficient of 0.997. The method was validated for specificity, linearity, limit of detection, limit of quantification, precision, robustness and stability. All the validation parameters were within the compendial requirements. The proposed method was successfully adopted for the analysis of Citalopram Hydrobromide in bulk, pharmaceutical dosage forms and in dissolution samples.
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