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A total of 41 patients requested thawing of supernumerary embryos in an intracytoplasmic sperm injection (ICSI) programme. Mean patient age was 30.8 ⍨ 3.8 years. Embryo freezing by the ultrarapid method was performed at room temperature in 3 mol/l DMSO and 0.25 mol/l sucrose. Total freezing time was 2.5 min including filling of the straw. In the thawing process, the embryos were removed from liquid N 2 , left at room temperature for 30 s, immersed for 40 s at 30°C, and then successivelydoi:10.1093/humrep/14.9.2362 pmid:10469711 fatcat:ccgkjehhlba6thmmjtnefajfhy