Enhancement of serotonin1A receptor function following repeated electroconvulsive shock in young rat hippocampal neurons in vitro

Kumatoshi Ishihara, Taku Amano, Hiroshi Hayakawa, Shigeto Yamawaki, Masashi Sasa
1999 International Journal of Neuropsychopharmacology  
Effects of repeated electroconvulsive shock (ECS) treatment on 5-hydroxytryptamine (5-HT) response were investigated to elucidate the ECS-induced changes, which may be related to antidepressant effects, using electrophysiological methods with hippocampal slices in vitro. ECS was applied to Wistar rats once daily for 14 d from 3 wk of age (ECS group). Control animals did not receive ECS (control group). Twenty-four hours after the final ECS treatment, hippocampal slices were prepared for
more » ... repared for intracellular recording analysis. Application of 5-HT (0.1-30 µ) caused a dose-dependent hyperpolarization in hippocampal CA1 neurons. 5-HT-induced hyperpolarization in the ECS group was significantly greater than that in the control group. Furthermore, 8-OH-DPAT [8-hydroxy-2-(di-n-propylamino)tetralin], a 5-HT "A receptor agonist, also induced significantly larger hyperpolarization in the ECS group than in the control group. These results suggest that repeated ECS treatment enhances function of the 5-HT "A receptor for 5-HT. This supports the hypothesis that enhanced 5-HT "A receptor function, at least in part, contributes to the effectiveness of ECS treatment for depression directly and\or indirectly.
doi:10.1017/s1461145799001467 pmid:11281976 fatcat:lkdo7uvpofh4pmvndqs6ny5pzi