Molecular identification of endophytic fungi associated with Coleus forskohlii (Willd.) Briq

Grace Leena Crasta,, Koteshwar Anandrao Raveesha
2021 Journal of Applied Biology & Biotechnology  
Coleus forskohlii (Willd.) Briq. is a notable medicinal plant belonging to the family Lamiaceae. Understanding the diversity of endophytic fungi associated with this medicinally important plant species and screening them to yield bioactive compounds would be highly useful for the herbal drug industry. The present study was undertaken to isolate and identify the diversity of fungal endophytes associated with C. forskohlii (Willd.) Briq. The fungal endophytes were isolated following standard
more » ... dures and molecular identification was carried out by using the 18S rRNA gene; the amplified regions were sequenced and submitted to NCBI, GenBank. A total of 85 endophytic fungi were isolated from 280 leaf segments. Molecular identification revealed 34 fungal genera. Among these, species of Cladosporium sp., Alternaria sp., Aspergillus niger, Aspergillus sp., Colletotrichum sp., Nigrospora oryzae, Penicillium sp., and Phyllosticta fallopiae were found to be predominant genera. The percentage occurrence of members of Ascomycota was the highest, with 96.47% distribution and Basidiomycota members were distributed the least, with 3.53%. The study revealed the diversity of endophytic fungi associated with the leaves of C. forskohlii and the phylogenetic tree shows the relationships between the endophytic fungi. constitution, tissue type, microflora, and species type, as well as the external abiotic factors [2, 3] . Almost all parts of the plants, for instance, the roots, stem, leaves, rhizome, inflorescence, seeds, etc. are occupied by endophytic fungi [4] [5] [6] . These endophytic fungi act as a reservoir for novel bioactive metabolites such as terpenes, phenolics, alkaloids, tannins, saponins, steroids, and quinones which aid as potential drugs against microbes, insects, cancers, and have many more curative roles. Thus, recently, the endophytes associated with the plants rather than the plants themselves have emerged as promising mines for novel drug discovery. Therefore, studies on endophytes are now of great significance, not only to encounter the microbial multiplicity but also to obtain novel chemical compounds that could be assuring drugs to treat several ailments [7, 8] . The Mint family, known as Lamiaceae or Labiatae, encompasses 245 genera and 7,886 species. The major genera under Lamiaceae are Salvia (986 sp.), Scutellaria (486 sp.), Plectranthus (325 sp.),
doi:10.7324/jabb.2021.96021 fatcat:uskuibxnszfufnms6qjuzbx5y4