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Recently, it was suggested that the nitrite (NO2−) produced from NO3− by oral bacteria might contribute to oral and general health. Therefore, we aimed to clarify the detailed information about the bacterial NO2-production in the oral biofilm. Dental plaque and tongue-coating samples were collected, then the NO2-producing activity was measured. Furthermore, the composition of the NO2−-producing bacterial population were identified using the Griess reagent-containing agar overlay method anddoi:10.1038/s41598-020-73479-1 pmid:33024169 fatcat:rzjxdbxrsrgx3bjxfm7m3yebrm