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1 Microalgae are grown for the research on photosynthesis, biotechnology, and water-environment ecology. Specialized laboratories typically use calibrated commercial equipment, which agitates the microalgae culture by shaking or bubbling, controls the irradiance, temperature, and CO 2 content. Such commercial incubators may be out of reach for some laboratories, like those not specializing in microalgae research, teaching laboratories, or laboratories in low resource settings. Our research usesdoi:10.17504/protocols.io.8wuhxew fatcat:cn6ffq2nfzbe3dm4y6qeux5a2e