Recent Developments in Reliable and Reproducible Digital Immunogold Analysis in Electron Microscopy

J Krieger, M Bartels, W-J Chen, M Bode
2006 Microscopy and Microanalysis  
Immunocytochemical techniques have evolved rapidly in biological and medical research and are very important static methods for understanding interactions in the cell. Establishment of immunological methods using multiple antibodies for multiple epitopes together with multilabelling -using gold probes of different size in the same sample -is now possible for virtually every biological laboratory by using high quality protein-gold complexes and certain antibodies which are available commercially
more » ... ilable commercially in a broad range and in high-quality. Together with this possibility comes the need to analyze the results in the same high quality -reliably and reproducibly.
doi:10.1017/s143192760606483x fatcat:zqpvf2ntgja3bcuwhvrx3ckcla