The maintenance of a large collection of stock cultures of bacteria for ready availability is a major task. The method of frequent transfer on a suitable culture medium, as commonly employed, requires not only a great expenditure of time, materials and effort, but also involves the possible loss of certain biological, immunological and cultural characteristics; not to mention the able to a large and varied stock culture collection. It is our object to evaluate three representative methods by

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... ting them against most of the species commonly maintained 163 on May 8, 2020 by guest