Identification of Distinct Potyviruses in Mixedly-Infected Sweetpotato by the Polymerase Chain Reaction with Degenerate Primers

D. Colinet
1994 Phytopathology  
Colinet, D., Kummert, J., Lepoivre, P., and Semal, J. 1994. Identification of distinct potyviruses in mixedly-infected sweetpotato by the polymerase chain reaction with degenerate primers. 84:65-69. A combined assay of reverse transcription and the polymerase chain reaction utilizing degenerate primers derived from conserved regions in the genome of potyviruses was designed to amplify the variable 5'-terminal region of the coat protein cistron. Amplification on total RNA extracted GNNSGAP
more » ... acted GNNSGAP GNNSGOP GNNSGAP GNNSGOP GNNSGOP GNIISGOP
doi:10.1094/phyto-84-65 fatcat:7f4tjh6tqfhwbo7mh2zmdickjy