Original Article Clinicopathological significance and prognostic value of NKX2.2 expression in papillary thyroid carcinoma

Liang Hu, Zi-Li Yang, Yi-Jun Wu, Fu-Qiang Li, Qi-Han You
2017 Int J Clin Exp Pathol   unpublished
Objectives: This study aims to investigate NKX2.2 protein expressions in papillary thyroid carcinoma (PTC) patients, and discuss its correlation with PTC clinicopathological features and prognosis. Methods: A sum of 187 PTC patients treated in our hospital between October 2011 and September 2012 were randomly selected as the case group. Another 124 volunteers receiving health examination in our hospital were taken as the control group. A few thyroid cell groups were obtained by vivo fine needle
more » ... by vivo fine needle aspiration and fresh surgical PTC specimens were collected. NKX2.2 protein expressions in specimens were detected by immumohistochemical staining and Western Blot. Follow up was conducted for 3 years to study the relationship between NKX2.2 and postoperative recurrence rates. Results: Immunohistochemical staining showed that NKX2.2 protein showed different levels of expression in different staged PTC samples. Compared with the control group, positive ratios of NKX2.2 in the case group were significantly lower (all P < 0.05). As PTC worsened, positive ratios of NKX2.2 decreased. Western Blot showed that as PTC worsened, NKX2.2 protein expression declined. Maximum diameter, lymph node metastasis, distant metas-tasis and neoplasm invasiveness were negatively related to positive ratios of NKX2.2 and NKX2.2/GAPDH protein expression. Follow up analysis showed that as NKX2.2 protein expressions and positive ratios of NKX2.2 increased, postoperative recurrence rates declined. Conclusion: NKX2.2 protein is associated with PTC clinicopathological features and prognosis thus can be an indicator for PTC diagnosis and prognosis evaluation.