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Journal of Virology
Double-stranded, full-length linear DNA was synthesized in vitro by using single-stranded linear DNA as a self-priming template from the parvovirus Kilham rat virus and Escherichia coli DNA polymerase "large fragment" as the polymerizing enzyme. To ascertain the order of the synthesis of the cleavage fragments and to assess the accuracy of the in vitro synthesis, restriction endonuclease cleavage sites with kn6wn recognition sequences were mapped on the DNA. Comparing the cleavage pattern ofdoi:10.1128/jvi.27.3.784-790.1978 fatcat:23hq3hhn5vdc5lqzjc622pbwha