Various storage media have been used in orthodontic shear bond strength studies, however, the effect of the storage media on human enamel has not been studied. Aim: The purpose of this in vitro study was to determine the effect of four commonly used storage media on the shear bond strength values of orthodontic brackets bonded on extracted human teeth. Materials and methods: A total of 60 freshly extracted noncarious premolars teeth were randomly divided into four groups. Group 1 was stored in

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... istilled water, samples of group 2 were stored in 10% formalin, group 3 were preserved in 70% ethanol and samples of group 4 were stored in isotonic saline solution. After a storage period of 30 days, the teeth were rinsed and brackets bonded with Transbond XT and the specimens were evaluated for shear bond strength. Results: The 10% formalin sample had statistically significant greater bond strength than the other test groups. The 70% ethanol group had statistically lowest bond strength compared to other groups. The distilled water and isotonic saline solution group showed bond strength which was comparable. Conclusion: Storage media have an effect on bond strength results. Distilled water and isotonic saline storage produced comparable bond strength. Formalin and ethanol storage produced extreme variation in SBS values.