In VitroAntioxidant and Cytotoxic Activities ofArnebia benthamii(Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley

Showkat Ahmad Ganie, Tanveer Ali Dar, Rabia Hamid, Ovais Zargar, Shayaq Ul Abeer, Akbar Masood, Shajrul Amin, Mohammad Afzal Zargar
2014 Oxidative Medicine and Cellular Longevity  
Arnebia benthamiiis a major ingredient of the commercial drug available under the name Gaozaban, which has antibacterial, antifungal, anti-inflammatory, and wound-healing properties. In the present study,in vitroantioxidant and anticancer activity of different extracts ofArnebia benthamiiwere investigated. Antioxidant potential of plant extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. The highest
more » ... enolic content (TPC) of 780 mg GAE/g was observed in ethyl acetate, while the lowest TPC of 462 mg GAE/g was achieved in aqueous extract. At concentration of 700 µg/mL, DPPH radical scavenging activity was found to be highest in ethyl acetate extract (87.99%) and lowest in aqueous extract (73%). The reducing power of extracts increased in a concentration dependent manner. We also observed its inhibition on Fe2+/ascorbic acid-induced lipid peroxidation (LPO) on rat liver microsomesin vitro. In addition,Arnebia benthamiiextracts exhibited antioxidant effects on Calf thymus DNA damage induced by Fenton reaction. Cytotoxicity of the extracts (10–100 µg/mL) was tested on five human cancer cell lines (lung, prostate, leukemia, colon, and pancreatic cell lines) using the Sulphorhodamine B assay.
doi:10.1155/2014/792574 pmid:24734152 pmcid:PMC3964854 fatcat:nlz6df3vdnh6ll2ba6fn7xkmba