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Substrate channeling of oxalacetate in solid-state complexes of malate dehydrogenase and citrate synthase
Journal of Biological Chemistry
Current evidence suggests that mitochondrial matrix enzymes exist in solid-state, multienzyme complexes in vivo. Addition of polyethylene glycol to a solution containing malate dehydrogenase and citrate synthase generates such a solid-state, enzyme complex in vitro at enzyme concentrations permitting kinetic measurements. Suspensions of the isolated, solid-state, hetero-complex of these enzymes were used to study the coupled reactions of citrate synthesis from malate, NAD, and CoASAc. Thepmid:4066662 fatcat:7lfe6ni4m5d6hcxcesqcohk43a