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We present the clinical applicability of fluorescence ratio reference standard ͑FRRS͒ to discriminate different stages of dental caries. Toward this, laser-induced autofluorescence emission spectra are recorded in vivo in the 400-to 800-nm spectral range on a miniature fiber optic spectrometer from 65 patients, with a 404-nm diode laser as the excitation source. Autofluorescence spectra of sound teeth consist of a broad emission at 500 nm that is typical of natural enamel, whereas in cariesdoi:10.1117/1.3365945 pmid:20459276 fatcat:3y4s3jys35agndvw672bagvzty