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Leaf rust, caused by Puccinia triticina f. sp. tritici, is the most prevalent disease of wheat in Pakistan. Host resistance is the most effective and economical method of controlling the disease. In the present study, 254 Pakistani wheat germplasm were screened for the presence or absence of 17 leaf rust resistance (Lr) genes. Lr 09, 10, 20, 24, 25, 27, 28, 29, 35, 37, 39, 46, 47, 50, 51, 58 and Lr 61 were detected either as pyramided triplet and doublet genes, or as a single gene harboreddoi:10.36899/japs.2021.2.0235 fatcat:nkp5hjoo7zhf5mpkzsatowzmiq