Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues [article]

Anita Badbaran, Reiner Mailer, Christine Dahlke, Jannis Woens, Anahita Fathi, Sibylle C. Mellinghoff, Thomas Renné, Marylyn M. Addo, Kristoffer Riecken, Boris Fehse
2021 bioRxiv   pre-print
AbstractVaccination with the adenoviral-vector based Astra Zeneca ChAdOx1 nCov-19 vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector in vivo represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia
more » ... TT). We previously have shown that digital PCR is an excellent tool to quantify transgene copies in vivo. Here we present a highly sensitive digital PCR for in-situ quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human serum 24, 72 and 168 hours post vaccination, and in a variety of murine tissues in an experimental vaccination model 30 minutes post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.
doi:10.1101/2021.05.28.446155 fatcat:66f57ztowfb4ngh4xmksh6t5r4