Bacterial Biofilm Presence in Pediatric Tracheotomy Tubes

Jonathan Perkins, Jason Mouzakes, Rodrigo Pereira, Scott Manning
2004 Archives of Otolaryngology - Head and Neck Surgery  
Objective: To determine whether bacterial biofilms are present on pediatric tracheotomy tubes. Design: Prospective observational series. Interventions: Eleven tracheotomy tubes removed during routine tracheotomy tube changes were analyzed for biofilm and live bacteria presence using confocal microscopy and vital stains. The external and internal surfaces of the tracheotomy tubes were studied in 3 locations: distal tip, midtracheotomy tube, and proximal opening. These data were correlated with
more » ... e correlated with tracheotomy site cultures and the reason for tracheotomy dependence. Main Outcome Measures: Microscopic images were analyzed for the presence of a biofilm (its morphological features and the presence of live and dead bacteria within the biofilm).
doi:10.1001/archotol.130.3.339 pmid:15023844 fatcat:ysimrmqvgfevpo5vx7q736buxa